Acetylcholinesterase (AChE) is mainly involved in synaptic transmission by hydrolyzing acetylcholine in the synaptic cleft. It has been suggested that it could also be involved in other functions such as cell-cell adhesion. In this study, we have expressed mouse G2-GPI AChE at the membrane surface of S180 cells. We obtained a transfected cell line which permanently expresses high levels of AChE at the cell surface. However, transfected cells behave as single cells in culture. We performed cell aggregation and adhesion tests and found no significant aggregation or adhesion, which suggests that AChE is not a homophilic adhesion molecule.